Learn more about our New BaseScopeTM assay

Control Probes for Manual Assays: RNAscope 2.5 HD Duplex Reagent Kit

Species-specific positive probes and negative control probes are needed to run with Human or mouse control slides for technique quality control check and with your sample for Sample or RNA quality control check. ACD provides positive control probes that target species-specific housekeeping genes and negative control probes that target bacteria DapB gene.

Careful selection of the right positive control is recommended.  The expected expression level of your target RNA is the key criteria consider as your positive control probe should have similar expression profile.  See the overview page for details. 

Sample Images
RNAscope<sup>®</sup> 2.5 HD Duplex Assay shows expression of PPIB (Green) and Polr2a (red) in lung FFPE tissue

RNAscope® 2.5 HD Duplex Assay shows expression of PPIB (Green) and Polr2a (red) in lung FFPE tissue

RNAscope<sup>®</sup> 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in kidney FFPE tissue

RNAscope® 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in kidney FFPE tissue

RNAscope<sup>®</sup> 2.5 HD Duplex Assay shows expression of POL2A (Green) and TBP (red) in kidney FFPE tissue

RNAscope® 2.5 HD Duplex Assay shows expression of POL2A (Green) and TBP (red) in kidney FFPE tissue

RNAscope<sup>®</sup> 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in human Hela cells.

RNAscope® 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in human Hela cells.

RNAscope<sup>®</sup> 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in colon FFPI tissue.

RNAscope® 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in colon FFPI tissue.

RNAscope<sup>®</sup> 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in brain FFPE tissue.

RNAscope® 2.5 HD Duplex Assay shows expression of PPIB (Green) and POLR2A (red) in brain FFPE tissue.

When working with RNAscope®  2.5 HD Duplex chromogenic assay, you will need one premixed duplex positive control probe and one premixed duplex negative control probe..

RNAscope® 2.5 Duplex Positive Control Probe

Human (Cat. No. 321641), Mouse (Cat. No. 321651) 

Each control probe set contains premixed and ready to use PPIB probe in channel 1 and POLR2A probe in channel 2. Probe volume is configured for 20 reactions. 

RNAscope® 2-plex Negative Control Probe

(Cat. No. 320751)

Contains premixed probe for DapB in both channel 1 and 2. Probe volume is configured for 20 reactions.

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.

Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com